نوع مقاله : مقاله کامل پژوهشی
نویسندگان
1 دانشگاه کردستان. دانشکده کشاورزی
2 گروه آموزشی گیاهپزشکی، دانشکده کشاورزی، دانشگاه کردستان
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Because the mutant mlo allele causes a non-race specific and broad-spectrum resistance to powdery mildew caused by Blumeria graminis f. sp. hordei, the Mlo gene was taken into consideration in barley. Mlo genes also play important roles in plant growth and responses to biotic and abiotic stresses. The Mlo gene family has been studied in several plant species. In this study, we used bioinformatics tools and searches in genomic databases to reveal the genetic characteristics and protein structure of the Mlo family in wheat (Triticum aestivum) and apple (Malus domestica). We employed Mlo proteins sequences of Arabidopsis thaliana as a template for tBLASTn, which eventually identified 29 MdMlo (M. domestica Mlo) proteins and 11 TaMlo proteins (T. aestivum Mlo). The comparative phylogenetic analysis classified MdMlo and TaMlo proteins into three main clades and showed that, regardless of plant species, Mlo1s (the first member of the Mlo protein family in wheat, apple and Arabidopsis), Mlo2s, Mlo3s, and so on up to Mlo12s, are closely related. This indicates that after the separation of these species, no further expansion has been occurred in the Mlo gene family. The functionally conserved motifs present in the Mlo proteins were investigated using MEME tool, which showed a maximum of 15 and minimum 10 conserved motifs. The genes TaMlo6, MdMlo8, MdMlo11, TaMlo2, and TaMlo1 are predicted to participate in powdery mildew resistance because of having E/D-F-S-F motif.
کلیدواژهها [English]
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