ارزیابی واکنش چند ژنوتیپ پرشین لایم به‘Candidatus Phytoplasma aurantifolia’ در شرایط گلخانه

نوع مقاله : مقاله کامل پژوهشی

نویسندگان

1 دانشگاه تبریز - دانشکده کشاورزی - گروه گیاهپزشکی

2 دانشیار گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه تبریز، ایران.

3 موسسه مرکبات رامسر

4 عضو هیات علمی بخش تحقیقات گیاهپزشکی- مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی هرمزگان

چکیده

در این پژوهش واکنش سه ژنوتیپ متحمل شامل TLC876، TLCRO، TLCR10 و یک ژنوتیپ حساس (ژنوتیپ RA) پرشین‌لایم با روش مایه‌زنی با پیوند به فیتوپلاسمای جاروک لیموترش در شرایط گرم (دمای روز 37-35 و دمای شب 27-25 درجه‌ی سانتی‌گراد) ارزیابی شد. هم چنین، در شرایط دمایی خنک (دمای روز 26-24 درجه‌ی سانتی‌گراد و دمای شب 20-18 درجه‌ی سانتی‌گراد) واکنش ژنوتیپ RA به این بیمارگر بررسی شد. غلظت فیتوپلاسما در گیاهان مایه‌زنی‌شده در فواصل زمانی مشخص با استفاده از واکنش زنجیره-ای پلیمراز در زمان واقعی تعیین شد. چهل و هشت هفته بعد از مایه‌زنی، میانگین غلظت فیتوپلاسمای جاروک لیموترش در ژنوتیپ‌های TLCRO، TLC876 و TLC10 بسیار اندک و به ترتیب 17، 10 و 12 نسخه از ژنوم در 100 نانوگرم دی‌ان‌ای گیاه بود و علائمی از بیماری در آن‌ها مشاهده نشد. در ژنوتیپ RA، میانگین تعداد نسخه های فیتوپلاسمای جاروک لیموترش در 100 نانوگرم دی‌ان‌ای گیاه در شرایط گرم بیشتر از شرایط خنک بود و اختلاف معنی‌داری (P < 0.01) را نشان داد. علائم بیماری جاروک در پرشین لایم RA، فقط در شرایط گرم پدیدار شد و در شرایط خنک علائمی از بیماری مشاهده نشد. نتایج این پژوهش نشان داد که ژنوتیپ‌هایTLC10 ،TLC876 و TLCRO تحمل بالایی به ‘Ca. P. aurantifolia’ دارند و می-توانند به عنوان یک رقم متحمل یا مقاوم به بیماری معرفی شوند و نیز در برنامه‌های اصلاح ژنتیکی به منظور تولید ارقام مقاوم و متحمل مورد استفاده قرار گیرند. همچنین افزایش دما ممکن است میزان حساسیت برخی ژنوتیپ‌های پرشین لایم به فیتوپلاسمای بیماری جاروک لیموترش را افزایش دهد.

کلیدواژه‌ها


عنوان مقاله [English]

Reaction of some Persian lime genotypes to ‘Candidatus Phytoplasma aurantifolia’ under greenhouse condition

نویسندگان [English]

  • Sina Nourizadeh 1
  • R. Khakvar 2
  • Morteza Gholmohammadi 3
  • Seyyed Mahdi Bani Hashemian 3
  • Mohammad Mehdi Faghihi 4
1 Plant Protection Department, Faculty of Agriculture, university of Tabriz
2 Department of Plant Protection, Faculty of Agriculture, University of Tabriz
3 Citrus institute, Ramsar
4 Hormozghan
چکیده [English]

Lime witches' broom disease associated with ‘Candidatus Phytoplasma aurantifolia’ is one of the most serious threats to lime production in Oman, United Arab Emirates and Iranian southern provinces. In this study, under warm condition (35-37°C and 25-27°C day/night) the reaction of four genotypes of Persian lime including TLCR10, TLCRO, TLC876 (tolerant genotypes) and RA (sensitive genotype) to ‘Ca. P. aurantifolia’ was investigated via graft inoculation. Also, under cool condition (24-26°C and 18- 20°C day/night) using the same inoculation method, the reaction of RA genotype to this pathogen was evaluated. Quantitative PCR was used for detection and quantification of ‘Ca. P. aurantifolia’ in the inoculated plants. At 48 weeks post-inoculation, the copy numbers of ‘Ca. P. aurantifolia’ per 100 ng of total plant DNA in TLCRO, TLC876 and TLCR genotypes was very low (17, 10 and 12, respectively) and no disease symptoms were observed. In RA genotype under warm condition, the mean copy numbers of ‘Ca. P. aurantifolia’ per 100 ng of plant total DNA was higher than that of cool condition and showed significant differences (P < 0.01) . Furthermore, the disease symptoms were only appeared under the warm condition. The results of the present study showed that TLC10, TLC876 and TLCRO genotypes had high tolerance to ‘Ca. P. aurantifolia’ and can be introduced as tolerant or resistant cultivars to ‘Ca. P. aurantifolia’. These tolerant genotypes can be used in genetic breeding programs to produce resistant and tolerant cultivars. The results also indicated that higher temperature may change susceptibility of

کلیدواژه‌ها [English]

  • Lime witches’ broom
  • resistance
  • Real time PCR
  • Warm and cool conditions
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