جایگاه فیلوژنتیکی باکتری اندوفیت Bacillus sp. Bp91 جدا شده از سیب زمینی و فعالیت ضدمیکروبی آن علیه باکتری Ralstonia solanacearum

نوع مقاله : مقاله کامل پژوهشی

نویسندگان

1 دانشجوی دکتری بیماری‌شناسی گیاهی دانشکده علوم کشاورزی و صنایع غذایی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.

2 دانشیار گروه گیاه‌پزشکی، دانشکده علوم کشاورزی و صنایع غذایی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.

3 دانشیار گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه کردستان، سنندج، ایران.

4 دانشیار گروه گیاهپزشکی دانشکده کشاورزی دانشگاه رازی- کرمانشاه- ایران

چکیده

بیماری پژمردگی باکتریایی سیب‌زمینی ناشی از Ralstonia solanacearum یکی از مخرب-ترین بیماری‌های سیب‌زمینی در سراسر جهان و از جمله در استان کردستان ایران است. استفاده از باکتری‌های اندوفیت یکی از راهکارهای مؤثر در کنترل بیماری مذکور است. در این تحقیق از سویه Bacillus sp. Bp91 که به‌عنوان اندوفیت هم‌ستیز برتر از مزارع سیب زمینی استان کردستان جداسازی شده بود برای آزمون‌های تکمیلی استفاده شد. نتایج آزمون‌های بررسی مکانیسم بازدارندگی نشان داد تولید پروتئاز در سویه Bacillus sp. Bp91 مثبت و برای آزمون های سیانید هیدروژن و سیدروفور به ترتیب منفی و مثبت ضعیف بود. جایگاه تبارزایی سویه Bacillus sp. Bp91 نشانگر شباهت بسیار نزدیک به گونه‌های شناخته شده جنس Bacillus بویژه B. pulmilus می‌باشد. همچنین بررسی‌های متابولومیکسی توسط دستگاه GC-mass نشان‌‌داد دو متابولیت Eicosane و Dibutyl phthalate از شاخص‌ترین متابولیت‌های ضدمیکروبی باکتری مذکور می-باشند.

کلیدواژه‌ها


عنوان مقاله [English]

Phylogenetic status of the endophyte Bacillus sp. Bp91 isolated from potato and its antibacterial activity against Ralstonia solanacearum

نویسندگان [English]

  • Kambiz Bahmani 1
  • nader hasanzadeh 2
  • Behrouz Harighi 3
  • Alireza Marefat 4
1 Department of Plant Protection, Faculty of Agricultural Sciences and Food Industries, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2 Department of Plant Protection, Faculty of Agricultural Sciences and Food Industries, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 Department of Plant Protection, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran.
4 Department of Plant Protection, Faculty of Agriculture, Razi University, Kermanshah, Iran.
چکیده [English]

Bacterial wilt disease caused by Ralstonia solanacearum is one of the most destructive diseases in the world, including Kurdistan province of Iran. Using the endophytic bacteria is one of the most effective and applicable methods to control of the disease. In this study, Bacillus sp. Bp91 which was isolated from potato fields in Kurdistan province was used as a superior antagonist against R. solanacearum. The results of the supplementary tests showed that the production of protease in the mentioned strain is positive. This was negative and relatively weak for hydrogen cyanide and siderophore production, respectively. The phylogenetic tree derived from 16S rRNA gene sequence showed the stain belongs to the genus Bacillus sharing the highest level of sequence homology with to B. pumilus.
Metabolic studies by GC-mass were also indicated that the two metabolites Eicosane and Dibutyl phthalate are the most prominent antimicrobial metabolites produced by the bacterium.

کلیدواژه‌ها [English]

  • biological control
  • Endophytic Bacteria
  • Metabolite
  • Potato
  • GC-MS
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