برهمکنش فیتوپلاسماهای عامل بیماری‌های جاروک لیموترش و فیلودی کاهو در آلودگی مخلوط بوته‌های پروانش

نوع مقاله: مقاله کامل پژوهشی

نویسندگان

1 دانشجوی دکتری بیماری شناسی گیاهی، ، دانشکده کشاورزی، دانشگاه شیراز، ایران

2 استاد بخش گیاهپزشکی، دانشکده کشاورزی، دانشگاه شیراز، ایران

3 بخش گیاهپزشکی، دانشکده کشاورزی، دانشگاه شیراز، ایران

4 دانشیار بخش گیاهپزشکی، دانشکده کشاورزی، دانشگاه شیراز، ایران

چکیده

در این پژوهش اثر آلودگی مخلوط فیتوپلاسماهای جاروک لیموترش (16SrII-B) و فیلودی کاهو (16SrIX-D) بر روی علائم بیماری و تراکم  سلول‌های فیتوپلاسما در بوته‌های  پروانش (Catharanthus roseus (L.) G.Don) مطالعه شد. تعداد چهار بوته پروانش سالم  و هم‌سن  از طریق قطعات سس آلوده ابتدا با فیتوپلاسمای عامل فیلودی کاهو و پس از سه هفته با فیتوپلاسمای جاروک لیموترش مایه‌زنی شدند. علائم بیماری و جمعیت فیتوپلاسما در این بوته‌ها با علائم بیماری و جمعیت فیتوپلاسما در بوته‌هایی که تنها با یکی از این فیتوپلاسماها مایه‌زنی شده بودند مقایسه گردید. در بوته‌هایی که به صورت مخلوط مایه‌زنی شده بودند تنها کاهش خفیفی در اندازه برگ و فاصله میانگره‌ها ایجاد شد که با علائم شدید ناشی از هر یک از دو فیتوپلاسما، کاملا متفاوت بود. بوته‌هایی که به صورت مخلوط مایه‌زنی شده بودند در مقایسه با بوته‌هایی که تنها با یک فیتوپلاسما مایه‌زنی شده بودند طول عمر بیشتری داشتند. با استفاده از پی‌سی‌آر معمولی و آغازگرهای اختصاصی وجود هر دو فیتوپلاسما در بوته‌هایی که به صورت مخلوط مایه‌زنی شده بودند و همچنین در بوته‌های شاهد مثبت به اثبات رسید. آنالیز داده‌های حاصل از آزمون پی‌سی‌آر در زمان واقعی (Real-time PCR) نشان داد که جمعیت هر دو فیتوپلاسما در بوته‌هایی که به صورت مخلوط مایه‌زنی شده بودند در مقایسه با بوته‌های شاهد مثبت به طور معنی‌داری کاهش داشت. به نظر می‌رسد برهمکنش این دو فیتوپلاسما در آلودگی مخلوط از نوع آنتاگونیسم متقابل باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Interaction of witches’- broom disease of lime phytoplasma and lettuce phyllody phytoplasma in mixed infection of periwinkle plants

نویسندگان [English]

  • E. Salehi Abarghouei 1
  • K. Izadpanah 2
  • S. M. Taghavi 3
  • H. Hamzezarghani 4
  • A. Afsharifar 2
چکیده [English]

In this research the effect of mixed infection of witches’ broom disease of lime (WBDL) (16SrII-B) and lettuce phyllody (LP) (16SrIX-D) phytoplasmas on symptom expression and phytoplasma density  in periwinkle plants (Catharanthus roseus (L.) G. Don) were investigated. Four healthy periwinkle plants of the same age were first inoculated with LP phytoplasma and after three weeks with WBDL phytoplasma using detached fragments of inoculative dodder (Cuscuta campestris Yank.). Disease symptoms and phytoplasma population in plants inoculated with both phytoplasmas were compared with those in plants inoculated with either WBDL or LP phytoplasma. In doubly inoculated periwinkle plants the disease symptoms mitigated to mild reduction of leaf size and internodes which strongly differed from those in periwinkle plants singly inoculated with either WBDL or LP phytoplasma. Plants with mixed infection lived longer than those inoculated singly with either phytoplasma. Both WBDL and LP phytoplasmas could be detected in singly and doubly infected plants by conventional and real time PCR assays using specific primer pairs. Analysis of the data obtained by real-time PCR showed that the population of both WBDL and LP phytoplasmas in the mixed infection was significantly lower than that in plants singly inoculated with WBDL or LP phytoplasma. It seems that interaction of WBDL and LP phytoplasmas in mixed infection of periwinkle is of the mutual suppression type.

کلیدواژه‌ها [English]

  • Mixed infection
  • Mutual antagonism
  • Phytoplasma population
  • Real-time PCR
 

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