عنوان مقاله [English]
نویسنده [English]چکیده [English]
Cereal yellow dwarf viruses are among the most destructive cereal viruses worldwide. In this study, subcellular localizations of silencing suppressor proteins ( P0) from two cereal yellow dwarf viruses, Cereal yellow dwarf virus-RPV (CYDV-RPV) and Cereal yellow dwarf virus-RPS (CYDV-RPS), were examined via confocal microscopy. To do so, P0 genes were synthesized by polymerase chain reaction (PCR) and were cloned through Gateway cloning system. Agrobacterium. tumefaciens clones harboring GFP:P0 constructs were infiltrated into Nicotiana benthamiana plants. Transient expression of P0 proteins with N-terminal GPF fusion enabled us to examine their subcellular localizations in plant cells, 24 hours post infiltration. Results showed that both proteins are nuclear-cytoplasmic but different tendencies were observed in their localizations. In other words, P0 of CYDV-RPS was more nuclear oriented than P0 of CYDV-RPV. In the Western blot analysis using GFP polyclonal antibody only GFP:P0 proteins but no free GFP, were detected indicating that localizations were related to GFP:P0 proteins.