اثر بیمارگر قارچی Leptosphaeria maculans در مرحله زیواپرور بر پروفیل متابولیکی گیاه کلزا رقم هایولا 401 در یک برهم کنش سازگار

نوع مقاله: مقاله کامل پژوهشی

نویسندگان

چکیده

پیشرفت­های اخیر در زمینه ابزارها و استخراج داده­ها، امکان مطالعه تاثیر تنش­های مختلف را بر متابولیسم اولیه و ثانویه گیاه به­وجود آورده است. مطالعه مکانیسم­های بیماریزایی و شناسائی مسیرهای متابولیکی مرتبط با بیماریزائی در گیاه، تغییرات در پروفیل متابولیکی بخش قطبی حاصل از برهمکنش سازگار قارچ Leptosphaeria maculans (فرم غیرجنسی Phoma lingam) (جدایه گرگان) و کلزا رقم حساس هایولا 401 به کمک دستگاه کروماتوگرافی گازی-طیف­سنج جرمی (GC-MS) مورد بررسی قرار گرفت. بدین منظور در شرایط کاملاً کنترل شده، 14 روز پس از کشت، در مرحله دو لپه­ای، گیاهان به ترتیب با سوسپانسیون 107 اسپور در میلی­لیتر و آب (شاهد) به روش قطره-زخم مایه­زنی شدند. 48 ساعت پس از مایه­زنی نمونه­برداری بافت محل مایه­زنی انجام شد و به سرعت تحت شوک سرمایی قرار گرفت. متابولیت‌ها با حلال متانول استخراج و به کمک دستگاه GC-MS شناسایی و اندازه‌گیری شده و مورد تجزیه و تحلیل آماری قرار گرفتند. تعداد 70 متابولیت نسبت به شاهد خود تغییرات معنی‌داری (P ≤0.05) نشان دادند که منجر به تغییرات در 28 مسیر متابولیکی در سلول‌های گیاه بود. در این برهمکنش تعدادی مسیر از مسیرهای متابولیکی دخیل در تقویت دیواره­ی سلولی، تولید ترکیبات فنولی، آلکالوئیدی، ترپنوئیدی، تولید انرژی، تولید هورمون و انتقال سیگنال­ تضعیف گردید. همچنین فراوانی تعداد زیادی از قندها کاهش یافت که این موضوع نقش حیاتی این کربوهیدرات­ها را در بیماریزایی P. lingamدر برهم کنش سازگار با یک رقم حساس پیشنهاد می­کند.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of fungul pathogen Leptosphaeria maculans at biotrophic stage on canola metabolic profile cultivar Hyola 401 in a compatible interaction

نویسندگان [English]

  • Z. Amjadi
  • H. Hamzehzarghani
چکیده [English]

Recent improvements in instrumentation and data mining have made it possible to investigate the effects of various stressors on primary and secondary metabolisms. In this study, in order to study mechanisms of pathogenesis and identify the pathogenesis-related metabolic pathways, changes in metabolic profiles resulting from a compatible interaction between canola cultivar (Hyola 401) and Leptosphaeria maculans (anamorph Phoma lingam) (Phkv102 isolate) were investigated by GC-MS chromatography using the polar fraction of plant extract. Under completely controlled conditions, 14 days after planting, when cotyledons were completely unfolded, the plants were inoculated by 107 spores per ml suspensions and water (control) by drop-wound method, respectively. The tissue samples were taken 48 hours after inoculation and were shock frozen immediately. Metabolites were extracted using methanol as solvent, identified and quantified with GC-MS and subjected to statistical analyses. Results indicated that 70 metabolites showed significant difference over their controls at P≤0.05, which is caused by changes in 28 metabolic pathways. Infection of canola cultivar Hyola 401 to P. lingam resulted in change in pathways related to the host cell wall, phenolic compounds, alkaloids and terpenoids biosyntesis, energy generator, hormone biosynthesis, signal transduction.The abundance of a number of sugars was also decreased, suggesting the crucial role of these carbohydrates in pathogenesis in an incompatible interaction of  P. lingam and a susceptible cultivar.

کلیدواژه‌ها [English]

  • Pathogenesis mechanisms
  • Susceptible cultivar
  • Metabolic pathways

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