تعیین توالی کامل جدایه ایرانی ویروس موزاییک چغندرقند

نوع مقاله: مقاله کامل پژوهشی

نویسندگان

چکیده

ویروس موزاییک چغندر (Beet mosaic virus, BtMV)، تنها پوتی‌ویروس آلوده‌کننده چغندرقند بوده و امروزه در سراسر چغندرکاری‌های جهان پراکنش دارد. در مطالعه حاضر ژنوم کامل جدایه‌ای از این ویروس (Ir-VRU) با استفاده از آغازگرهای دژنره عمومی پوتی‌ویروس‌ها، آغازگرهای اختصاصی طراحی شده و همچنین روش 5'-RACE برای اولین بار در ایران توالی یابی شد. ژنوم کامل جدایه ایرانی BtMV، بدون در نظر گرفتن دنباله poly-A، 9591 باز طول داشته، و بخش‌های 5'-UTR و 3'-UTR به ترتیب 165 و 168 باز می‌باشند. موتیف‌های معمول، چارچوب ژنی پلی‌پروتئین و PIPO در این جدایه شناسایی گردید. کد شدن پلی‌پروتئینی به طول 3086 آمینواسید و نه جایگاه برش در این پلی‌پروتئین پیش بینی گردید. بربررسی‌ها بر روی پنج جدایه مختلف BtMV نشان داد که جدایه ایرانی یکسانی زیادی به دو جدایه آلمانی و چینی داشته و از طرف دیگر بیشترین تفاوت را با جدایه آمریکایی نشان می‌دهد. تحلیل تبارزائی نیز جدایه Ir-VRU را به‌عنوان یک عضو از سوپر گروه BCMV نشان می‌دهد.

کلیدواژه‌ها


عنوان مقاله [English]

Full genome sequencing of Iranian isolate of Beet mosaic virus

نویسندگان [English]

  • M. Mohammadi
  • A. Hosseini
چکیده [English]

Beet mosaic virus (BtMV), the only potyvirus infecting beets, is distributed worldwide in beet growing areas. Here we report the full-length genome sequence of a BtMV isolate from Iran (Ir-VRU), which has enabled us to better understanding the evolutionary history of the virus. The complete nucleotide sequence of Ir-VRU genome was determined using degenerate and specific primers and 5'-RACE and The complete genomes consisted of 9591 nucleotides, excluding the poly-A tails. The genome has a 165 nt 5´-UTR and a 168 nt 3´-UTR. The RNA of Ir-VRU potentially encodes a single polyprotein of 3086 amino acid residues and has a deduced genome organization typical for members of the genus Potyvirus. Study on five different BtMV isolates showed that Iranian isolate closelly related to German and Chinese isolates, and on the other hand, it showed more differences with American isolate. Phylogenetic analysis, along with other potyviruses, also presents the Ir-VRU as a member of the BCMV supergroup.

کلیدواژه‌ها [English]

  • sugar beet
  • Beet mosaic virus
  • Phylogentic
  • potyvirus

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