برآورد شدت بیماری ناشی از ویروس وای سیب‌زمینی بر اساس وقوع بیماری در مزارع توتون استان گلستان

نوع مقاله : مقاله کامل پژوهشی

نویسندگان

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چکیده

ویروس وای سیب­زمینی (Potato virus Y, PVY)، گونۀ تیپ جنس Potyvirusاز تیرة Potyviridae، از جمله ویروس­های مخرب­ توتون در دنیا می­باشد. به منظور تعیین رابطه کمی بین میزان وقوع و شدت بیماری ناشی از PVY در پنج رقم رایج مورد کشت در مزارع توتون استان گلستان، طی فصل زراعی 1396، تعداد 15 مزرعه توتون در سه شهرستان گرگان، علی­آباد و مینودشت انتخاب و در فواصل زمانی 15 روزه مورد بازدید قرار گرفت. در هر مزرعه 50 بوته انتخاب و شاخص­های وقوع و شدت بیماری در آنها ارزیابی گردید. به منظور ردیابی ویروس از روش DAS-ELISA با آنتی­سرم چند همسانه­ای اختصاصی PVY استفاده شد. درصد آلودگی PVY در بین ارقام مختلف توتون متفاوت بوده و بیشینه و کمینه درصد آلودگی به تریتب در ارقام PVH03 (6/33 %) و بارلی (2/6 %) ارزیابی شد. بر اساس نتایج حاصل از تحلیل آماری داده­ها، بین ارقام توتون در شاخص­های مورد ارزیابی تفاوت معنی­دار بوده و بیشینه و کمینه میانگین شدت علایم در بوته­های آلوده به PVY، به ترتیب در ارقام PVH03 (44/4) و بارلی (82/1) مشاهده شد. همچنین مدل ریشه­مربع (در حدود 60 درصد مزارع) با ضریب تبیین متوسط 32/82 درصد و آلومتری (در حدود 30 درصد مزارع) با ضریب تبیین متوسط 90/89 درصد، به عنوان بهترین مدل جهت توصیف روابط بین شاخص­های وقوع و شدت بیماری در مزارع توتون انتخاب شدند. ارقام توتون بر اساس تجزیه­ی واریانس شیب خط رگرسیون مدل ریشه­مربع بین شاخص­های وقوع و شدت بیماری در سه گروه: رقم PVH03 با شیب بالا (005/1)، رقم NC100 با شیب متوسط (729/0) و ارقام K326، بارلی و باسما با شیب کم (68/0-6/0) قرار گرفتند.

کلیدواژه‌ها


عنوان مقاله [English]

Estimation of disease severity of potato virus y based on disease incidence in Golestan province tobacco fields

نویسندگان [English]

  • P. Sharifi Nezamabad
  • S. Nasrollah Nejad
  • A. Dizadji
  • M. A. Aghajani
  • A. Nadimi
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چکیده [English]

Potato virus Y, the type member of the genus Potyvirus, family Potyviridae, is one of the most destructive viruses of tobacco worldwide. To determine the relationships between incidence (I) and severity (S) of PVY disease in five cultivars in tobacco fields in Golestan province, several surveys were conducted during July to October 2017 at 15 days intervals in tobacco fields of Gorgan, Aliabad and Minoodasht. Two disease indicators (I and S) were evaluated in 50 plant samples for each farm. The presence of PVY was detected by double antibody sandwich-ELISA (DAS-ELISA) using PVY-specific polyclonal antibodies. The rate of PVY infection was different among tobacco cultivars and the maximum and minimum infection rates were belonged to PVH03 (33.6%) and Burley (6.2%) cultivars respectively. Based on the statistical analysis of data, there was a significant difference for disease indicators on different tobacco cultivars, as the maximum and minimum symptom severity mean value were evaluated in PVH03 (4.44) and Burley (1.82) cultivars, respectively. Also, Square Root model (Sqrt transformation of I and S) in 60 % of the fields (R2 = 82.32 %), and Allometric model (natural logarithm transformation of I and S) in 30 % of the fields (R2 = 89.90 %) showed the best fitness for the collected data, andwere the best model to describe I-S relationships in PVY disease in tobacco fields. Based on the regression slope of Sqrt transformed values of I and S, the cultivars were classified in three groups, including PVH03 (with the highest slope of 1.005), NC100 (with the moderate slope of 0.729) and K326, Burley and Basma (with thelowest slope of 0.6 – 0.66).

کلیدواژه‌ها [English]

  • Allometric model
  • PVH03 tobacco cultivar
  • R square
  • Severity symptom
  • Square Root model

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