برآورد شدت بیماری ناشی از ویروس وای سیب‌زمینی بر اساس وقوع بیماری در مزارع توتون استان گلستان

نوع مقاله: مقاله کامل پژوهشی

نویسندگان

چکیده

ویروس وای سیب­زمینی (Potato virus Y, PVY)، گونۀ تیپ جنس Potyvirusاز تیرة Potyviridae، از جمله ویروس­های مخرب­ توتون در دنیا می­باشد. به منظور تعیین رابطه کمی بین میزان وقوع و شدت بیماری ناشی از PVY در پنج رقم رایج مورد کشت در مزارع توتون استان گلستان، طی فصل زراعی 1396، تعداد 15 مزرعه توتون در سه شهرستان گرگان، علی­آباد و مینودشت انتخاب و در فواصل زمانی 15 روزه مورد بازدید قرار گرفت. در هر مزرعه 50 بوته انتخاب و شاخص­های وقوع و شدت بیماری در آنها ارزیابی گردید. به منظور ردیابی ویروس از روش DAS-ELISA با آنتی­سرم چند همسانه­ای اختصاصی PVY استفاده شد. درصد آلودگی PVY در بین ارقام مختلف توتون متفاوت بوده و بیشینه و کمینه درصد آلودگی به تریتب در ارقام PVH03 (6/33 %) و بارلی (2/6 %) ارزیابی شد. بر اساس نتایج حاصل از تحلیل آماری داده­ها، بین ارقام توتون در شاخص­های مورد ارزیابی تفاوت معنی­دار بوده و بیشینه و کمینه میانگین شدت علایم در بوته­های آلوده به PVY، به ترتیب در ارقام PVH03 (44/4) و بارلی (82/1) مشاهده شد. همچنین مدل ریشه­مربع (در حدود 60 درصد مزارع) با ضریب تبیین متوسط 32/82 درصد و آلومتری (در حدود 30 درصد مزارع) با ضریب تبیین متوسط 90/89 درصد، به عنوان بهترین مدل جهت توصیف روابط بین شاخص­های وقوع و شدت بیماری در مزارع توتون انتخاب شدند. ارقام توتون بر اساس تجزیه­ی واریانس شیب خط رگرسیون مدل ریشه­مربع بین شاخص­های وقوع و شدت بیماری در سه گروه: رقم PVH03 با شیب بالا (005/1)، رقم NC100 با شیب متوسط (729/0) و ارقام K326، بارلی و باسما با شیب کم (68/0-6/0) قرار گرفتند.

کلیدواژه‌ها


عنوان مقاله [English]

Estimation of disease severity of potato virus y based on disease incidence in Golestan province tobacco fields

نویسندگان [English]

  • P. Sharifi Nezamabad
  • S. Nasrollah Nejad
  • A. Dizadji
  • M. A. Aghajani
  • A. Nadimi
چکیده [English]

Potato virus Y, the type member of the genus Potyvirus, family Potyviridae, is one of the most destructive viruses of tobacco worldwide. To determine the relationships between incidence (I) and severity (S) of PVY disease in five cultivars in tobacco fields in Golestan province, several surveys were conducted during July to October 2017 at 15 days intervals in tobacco fields of Gorgan, Aliabad and Minoodasht. Two disease indicators (I and S) were evaluated in 50 plant samples for each farm. The presence of PVY was detected by double antibody sandwich-ELISA (DAS-ELISA) using PVY-specific polyclonal antibodies. The rate of PVY infection was different among tobacco cultivars and the maximum and minimum infection rates were belonged to PVH03 (33.6%) and Burley (6.2%) cultivars respectively. Based on the statistical analysis of data, there was a significant difference for disease indicators on different tobacco cultivars, as the maximum and minimum symptom severity mean value were evaluated in PVH03 (4.44) and Burley (1.82) cultivars, respectively. Also, Square Root model (Sqrt transformation of I and S) in 60 % of the fields (R2 = 82.32 %), and Allometric model (natural logarithm transformation of I and S) in 30 % of the fields (R2 = 89.90 %) showed the best fitness for the collected data, andwere the best model to describe I-S relationships in PVY disease in tobacco fields. Based on the regression slope of Sqrt transformed values of I and S, the cultivars were classified in three groups, including PVH03 (with the highest slope of 1.005), NC100 (with the moderate slope of 0.729) and K326, Burley and Basma (with thelowest slope of 0.6 – 0.66).

کلیدواژه‌ها [English]

  • Allometric model
  • PVH03 tobacco cultivar
  • R square
  • Severity symptom
  • Square Root model

Aghajani M.A. 2008. Epidemiologies of Sclerotinia stem rot of Canola in Golestan province. Ph. D. Thesis, Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.

Arabi M.I.E. and Jawhar M. 2010. Interrelationship between incidence and severity of leaf stripe on barley. Journal of Plant Pathology 92(2): 503-505.

Bhyan S.B., Alam M.M. and Ali M.S. 2007. Effect of Plant Extracts on Okra mosaic virus Incidence and Yield Related Parameters of Okra. Asian Journal of Agricultural Research, 1: 112-118. 10.3923/ajar.2007.112.118.

Boquel S., Giordanengo P., Ameline A. 2011. Divergent effects of PVY-infected potato plant on aphids. European Journal of Plant Pathology 129(4):507–510.

Boquel S., Delayen C., Couty A., Giordanengo P., Ameline A. 2012. Modulation of aphid vector activity by Potato virus Y on in vitro potato plants. Plant Disease 96:82–86.

Burgyán J., Havelda Z. 2011. Viral suppressors of RNA silencing. Trends Plant Science 16: 265–272.

Butt D.J., Royle D.J. 1980. The importance of terms and definitions for a conceptually unified epidemiology pp. 29-45. In: J. Palti and Kranz, J. (Eds). Comparative Epidemiology. A Tool for Better Disease Management. Centre for Agricultural Publishing and Documentation, Wageningen.

Campbell C.L. and Madden L.V. 1990. Introduction to plant disease epidemiology. John Wiley, New York, 532 pp.

Cardoso J.E., Santos A.A., Rossetti A.G. and Vidal J.C. 2004. Relationship between incidence and severity of cashew gummosis in semiarid north-eastern Brazil. Plant Pathology 53: 363-367.

Casteel C. L., Yang C., Nanduri A. C., De Jong H. N., Whitham S. A., Jander G. 2014. The NIa-Pro protein of Turnip mosaic virus improves growth and reproduction of the aphid vector, Myzus persicae (green peach aphid). Plant Journal 77:653–663.

Chen X.M., Liu J., Xu L., Jiang F., Xie X.Y., Zhu C.X. and Wen F.J. 2010. Inhibiting virus infection by RNA interference of the eight functional genes of the Potato Virus Y genome. Journal of Phytopathology 158 (11-12): 776-784.

Clark M.F. and Adams A.N. 1977. Characteristics of the microplate method of Enzyme-linked immunosrobent assay for the detection of plant viruses. Journal of General Virology 34:475-483.

Coresta Guide. 2009. A Scale for Coding Growth Stages in Tobacco Crops in Growth Stages of Mono- and Dicotyledonous- Plants’, BBCH Monograph, 2nd edition, 2001, (Eds). U, Meier, Federal Biological Research Centre for Agriculture and Forestry (nd www.bba.de/veroeff/bbch/bbcheng.pdf )

Culbreath A.K., and Srinivasan R. 2011. Epidemiology of spotted wilt disease of peanut caused by Tomato spotted wilt virusin the southeastern U.S. Virus Research 159:101-109.

Das Gupta I., Malathi V.G. and Mukherjee S.K. 2003. Genetic engineering for virus resistance. Current Science 84: 341-354.

Döring T.F. 2011. Potential and limitations of plant virus epidemiology: lessons from the Potato virus Y pathosystem. Potato Research 54:341-354.

El-Sawy M.M., Elsharkawy M.M., Abass J.M. and Kasem M.H. 2017. Antiviral Activity of 2-Nitromethyl Phenol, Zinc Nanoparticles and Seaweed Extract Against Cucumber mosaic virus (CMV) in Eggplant. Journal of Virology & Antiviral Research 6: (2).

Fargalla F.H., Taha A.M. and Fahim M.A. 2011. Epidemiology of Tomato yellow leaf curl virus in relation to intercropping and insecticidal spray effects on the Bemisia tabaci under field conditions. Acta Horticulturae 331- 336.

Fauquet C. and Fargette D. 1990. African cassava mosaic virus: etiology, epidemiology and control. Plant Disease 74: 404–411.

Gao S.J., Xia L.J., Liu Y. and Xie D.H. 2015. A plant virus disease model with periodic environment and pulse roguing. Studies in Applied Mathematics 136:357 381. 

Ghasemi M., Aghajani M. A., Faraji A. and R. Saidinejad M. 2013. Relationship between Incidence and severity of Alternaria blight disease on different species of brassica in Gonbad region. Iranian Journal of Plant Pathology 49(1): 17-19.

Giner A., Lakatos L., García-Chapa M., López-Moya J. J., Burgyán J. 2010. Viral protein inhibits RISC activity by Argonaute binding through conserved WG/GW motifs. A Peer-Reviewed Open-Access Journal 6, e1000996.

Guan J. and Nutteer F.W. J. R. 2003. Quantifying the interarater repeatability and interrater reliabilityof visual and remote-sensing disease-assessment methods in the alfalfa foliar pathosystem. Canadian Journal of Plant Pathology 25:143-149.

Harrison B.D., Zhou X., Otim-Nape G.W., Liu Y., Robinson D. J. 1997. Role of a novel type of double infection in the geminivirus-induced epidemic of severe cassava mosaic in Uganda. Annals of Applied Biology 131: 437–448.

Hosseini A. Sajjadi A and Mahdavi A. R. 2016. Evaluation the reaction of breeded male sterile air-cured tobacco hybrids to Potato virus Y and cucumber mosaic virus. Proceeding of the 22nd Iranian Plant Protection Congress, College of Agricultural and Natural Resources, University of Tehran, Karaj, IRAN, 17 p.

Ingwell L. L., Eigenbrode S. D., Bosque-Perez N. A. 2012. Plant viruses alter insect behavior to enhance their spread. International Journal of Scientific Reports 2:578.

James W.C. 1971. A manual of disease assessment keys for plant diseases. Canadian Department of Agricultural Publication 1450, 50p.

Jeger M. J., van den Bosch F., Madden L.V. and Holt J. 1998. A model for analysing plant-virus transmission characteristics and epidemic development. IMA journal of Mathematics Applied in Medicine and Biology 15:1-18.

Jeger M.J., Madden L.V. and van den Bosch F. 2018. Plant Virus Epidemiology: Applications and Prospects for Mathematical Modeling and Analysis to Improve Understanding and Disease Control. Plant Disease 102: 837-854.

Jurke C.J. and Fernando W.G.D. 2008. Effects of seeding rate and plant density on sclerotinia stem rot incidence in canola. Arch. Phytopat. Plant Protec 41:142-155.

Kerlan C. 2006. Potato virus Y. Descriptions of plant viruses, no. 414p. (http://www.dpvweb.net/ dpv/showdpv.php?dpvno=414)

Khateri H., Moarefzadeh N., Kohi-Habibi M., Mosahebi-Mohammadi Gh. and Hosseini A. 2004. Identification of viral diseases on commercial tobacco varieties in Mazandaran and Golestan regions, Research workbook of Plant protection department of Tirtash Research and Education Center.

Khateri H. 2015. Generation of PVY-resistant transgenic tobacco plants with using RNA-mediated gene silencing. Ph. D. Thesis, Department of Plant Pathology, Faculty of Agricultural and Natural Resources, University of Tehran, Karaj, IRAN.

Kirchner S.M. 2014. Epidemiology and enhance control of Potato virus Y in high grade seed potato production. Doctoral thesis in plant pathology, Faculty of Agriculture and Forestry, Department of Agricultura Sciences of the University of Helsinki. 167pp.

Kovač M., Muller A., Milovanovič J.D., Milavec M., Duchting P., Ravnikar M. 2009. Multiple hormone analysis indicates involvement of jasmonate signalling in the early defence of potato to Potato virus YNTN. Biologia Plantarum 53:195–199.

 Lacroix C., Glais L., Kerlan C., Verrier J.L. and Jacquot E. 2010. Biological characterization of French Potato virus Y (PVY) isolates collected from PVY-susceptible or -resistant tobacco plants possessing the recessive resistance gene va. Plant Pathology 59 (6): 1133-1143.

Lacomme C., Pickup J., Fox A., Glais L., Dupuis B., Steinger T., Rolot J.L., Valkonen J.P.T., Kruger K., Nie X., Modic S., Mehle N., Ravinkar M. and Hulle M. 2017. Transmission and Epidemiology of Potato virus Y, pp. 141–176. In: C. Lacomme, L. Glais, D.U. Bellsted, B. Dupuis, A.V. Karasev and E. Jaquot (Eds). Potato virus Y: biodiversity, pathogenicity, epidemiology and management Published by Springer Nature.

Legg J.P., Jeremiah S.C., Obiero H.M., Maruthi M.N., Ndyetabula I., Okao-Okuja G., Bouwmeester H., Bigirimana S., Tata-Hangy W., Gashaka G., Mkamilo G., Alicia T. and Kumar P.L. 2011. Comparing the regional epidemiology of the cassava mosaic and cassava brown streak virus pandemics in Africa. Virus Research159:161-170.

Luo Y.L., Gao S.J., Xie D.H. and Dai Y.F. 2015. A discrete plant disease model with roguing and replanting. Adv. Difference Equns. Article number 12. 

Madden L.V., Jeger M.J. and van den Bosch F. 2000. A theoretical assessment of the effects of vector-virus transmission mechanism on plant virus disease epidemics. Phytopathology 90:576-594.

McRoberts N., Hughes G. and Madden L. V. 2003. The theoretical basis and practical application of relationships between different disease intensity measurements in plants. Annals of Applied Biology 142: 191-211.

Moarefzadeh N. and Khateri H. 2009. Evaluation of burley tobacco varieties against of Potato virus Y, Research workbook of Plant protection department of Tirtash Research and Education Center.

Paul P.A., Lipps P.E. and Madden L.V. 2005. Relationship between visual estimates of Fusarium head blight intensity and deoxynivalenol accumulation in harvested wheat grain: A meta-analysis. Phytopathology 95:1225-1236.

Pita J.S., Fondong V.N., Sangaré A., Kokora R.N.N., Fauquet C.M. 2001. Genomic and biological diversity of the African cassava geminiviruses. Euphytica 120: 115–125.

Polhemus N.W. 1980. Interactive statistical graphics: system design criteria with an implementation in APL. Proceedings of the Statistical Computing Section, American Statistical Association, 391-396.

Powell G. 1991. Cell membrane punctures during epidermal penetrations by aphids: consequences for the transmission of two potyviruses. Annals of Applied Biology 119 (2): 313-321.

Rossel H.W., Asiedu R., Dixon A.G.O. 1992. Resistance of cassava to African cassava mosaic virus: what really pertains. Tropic. Root Tuber Crops Bull 6 (1): 2.

Sseruwagi P., Sserubombweb W.S., Legg J. P., Ndunguru J., Thresh J. M. 2004. Methods of surveying the incidence and severity of cassava mosaic disease and whitefly vector populations on cassava in Africa: a review. Virus Research 100: 129–142.

Schwanck A.A., Del Ponte E.M. 2014. Accuracy and reliability of severity estimates using linear or logarithmic disease diagram sets in true colour or black and white: a study case for rice brown spot. Journal of Phytopathology 162: 670–682.

Soitamo A. J., Jada B., Lehto K. 2011. HC-Pro silencing suppressor significantly alters the gene expression profile in tobacco leaves and flowers. BMC Plant Biology 11: 68.

Tsedaley B. 2015. A Review Paper on Potato Virus Y (PVY) Biology, Economic Importance and its Managements. Journal of Biology, Agriculture and Healthcare 5(9): 110-126.

Twengstrom E., Sigvald R., Svensson C. and Yuen J. 1998. Forecasting sclerotinia stem rot in spring sown oilseed rape. Crop Protection17: 405-411.

Valli A., Oliveros J. C., Molnar A., Baulcombe D., Garcia J. A. 2011. The specific binding to 21-nt double-stranded RNAs is crucial for the anti-silencing activity of cucumber vein yellowing virus P1b and per‌turbs small RNA populations. RNA 17: 1148–1158.

Velasco L., Simon B., Janssen D. and Cenis J.L. 2008. Incidence and progression of Tomato chlorosis virus disease and Tomato yellow leaf curl virus disease in tomato under different greenhouse covers in southeast Spain. Annals of Applied Biology 153:335-344. 

Verrier J.L., Marchand V., Cailleteau B. and Delon R. 2001. Chemical change andcigarette smoke mutagenicity increase associated with CMV and PVY infection inBurley tobacco. CORESTA Agronomy-PhytopathologyMeeting, Cape Town, SouthAfrica. P1.

Zadoks J.C. 1985. On the conceptual basis of crop loss assessment: The threshold theory. Annual Review of Phytopathology 23:455-473.

Zhou X., Liu Y., Calvert L., Munoz C., Otim-Nape G.W., Robinson D.J., Harrison B.D., 1997. Evidence that DNA-A of a geminivirus associated with severe cassava mosaic disease in Uganda has arisen by interspecific recombination. Journal of General Virology 78: 2101–2111.

Zhu Y. Y., H. Fang. Y. Y. Wang. J. X. Fan., Yang S. S. 2005. Panicle and canopy moisture in rice cultivar mixtures. Phytopathology 95: 433–438.