Identification of Cotton leaf curl Multan virus, a new threating Begomovirus in Iran

نوع مقاله : گزارش کوتاه

نویسندگان

1 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز، اهواز، ایران

2 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه شیراز

3 مرکز تحقیقات ملی ایتالیا، تورینو، ایتالیا

چکیده

Chinese hibiscus, Hibiscus rosa-sinensis L., is a common ornamental shrub in warm and humid regions of Iran like Khuzestan province, southwestern Iran. During 2019, some Chinese hibiscus plants showing begomovirus like symptoms including vein enation and leaf cupping (Figure 1A) were observed in Ahvaz, Khuzestan province. New plants obtained from cuttings of symptomatic plants showed the same symptoms. Leaf samples were collected and the extracted total DNA was subjected to polymerase chain reaction (PCR). Degenerate primer pairs were used to detect DNA A and DNA B genomic fragments of begomoviruses and their associated betasatellites. PCR resulted to amplify ~500 bp fragment of DNA A (complete V2 and partial V1 ORFs) while DNA B as well as betasatellite were not detected. Amplified fragments were purified and sequenced in both virion and complementary sense. Analyses performed using the Blastx program indicated that the DNA sequences have 93 to 96% pairwise identity with isolates of Cotton leaf curl Multan virus. Phylogenetic analysis revealed host preference between different analyzed begomovirus isolates so that cotton leaf curl viruses originated from H. rosa-sinensis like Khuzestan isolate of CLCuMuV formed a separated cluster. To our knowledge, this is the first report of CLCuMuV incidence in Iran. Considering the widespread presence of the begomovirus whitefly vector in the country, this virus that can be a serious threat to other economically important crops in Iran.

کلیدواژه‌ها

عنوان مقاله [English]

Identification of Cotton leaf curl Multan virus, a new threating Begomovirus in Iran

نویسندگان [English]

  • Nadia Mosharaf 1
  • Saeid Tabein 1
  • Seyed Ali Akbar Behjatnia 2
  • Gian Paolo Accotto 3
1 Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 Department of Plant Protection, School of Agriculture, Shiraz University, Shiraz, Iran
3 Institute for Sustainable Plant Protection, C.N.R., Torino, Italy
چکیده [English]

Chinese hibiscus, Hibiscus rosa-sinensis L., is a common ornamental shrub in warm and humid regions of Iran like Khuzestan province, southwestern Iran. During 2019, some Chinese hibiscus plants showing begomovirus like symptoms including vein enation and leaf cupping (Figure 1A) were observed in Ahvaz, Khuzestan province. New plants obtained from cuttings of symptomatic plants showed the same symptoms. Leaf samples were collected and the extracted total DNA was subjected to polymerase chain reaction (PCR). Degenerate primer pairs were used to detect DNA A and DNA B genomic fragments of begomoviruses and their associated betasatellites. PCR resulted to amplify ~500 bp fragment of DNA A (complete V2 and partial V1 ORFs) while DNA B as well as betasatellite were not detected. Amplified fragments were purified and sequenced in both virion and complementary sense. Analyses performed using the Blastx program indicated that the DNA sequences have 93 to 96% pairwise identity with isolates of Cotton leaf curl Multan virus. Phylogenetic analysis revealed host preference between different analyzed begomovirus isolates so that cotton leaf curl viruses originated from H. rosa-sinensis like Khuzestan isolate of CLCuMuV formed a separated cluster. To our knowledge, this is the first report of CLCuMuV incidence in Iran. Considering the widespread presence of the begomovirus whitefly vector in the country, this virus that can be a serious threat to other economically important crops in Iran.

کلیدواژه‌ها [English]

  • CLCuMuV
  • Begomovirus
  • Hibiscus

مراجع

Behjatnia S. A. A. and Karimi A. 2015. Etiology of Cotton Leaf Curl Disease in Fars Province. First International and 9th National Biotechnology Congress, Tehran, Iran.
Qadir R., Khan Z. A., Monga D. and Khan J. 2019. Diversity and recombination analysis of Cotton leaf curl Multan virus: a highly emerging begomovirus in northern India. BMC Genomics 20: 274-287.
Yazdani-Khameneh S., Aboutorabi S., Shoori M., Aghazadeh A., Jahanshahi P., Golnarahi A. and Maleki M. 2016. Natural Occurrence of Tomato leaf curl New Delhi virus in Iranian Cucurbit Crops. The Plant Pathology Journal 32: 201-208.
بیماریهای گیاهی
دوره 56، شماره 2
شهریور 1399
صفحه 217-218

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