نوع مقاله : مقاله کامل پژوهشی
عنوان مقاله [English]
Blast is a major disease of rice in Iran, causing severe damage in nursery and field. Piriformospora indica is a plant-root-colonizing fungus that can induce systemic resistance against different root and shoot pathogens. In this study expression rate of several defense genes in rice plant with or without P. indica treatment at different time points after inoculation with rice blast fungus (Magnaporthe oryzae) was evaluated using real-time qPCR. Rice seedlings were treated with mycorrhiza and spore suspension of the pathogen (M. oryzae) evaluation of the disease in plants treated and non-treated with M. oryzae and P.indica revealed that treated plants had less severe disease symptoms. Study on infection type, affected leaf area and number of lesions showed significant reduction of symptoms in treated plants compared to control. Also, expression amount of PR1b, LOX, NPR1 and WRKY85 genes increased in plants treated with mycorrhiza in comparison to control. Results of this study suggest the active role of mycorrhiza in protection of rice plant against blast disease due to enhanced expression of related defense genes.