Zucchini yellow mosaic virus (ZYMV (was isolated from squash samples with symptoms of stunting, leaf blistering and distortion of leaf and fruits, collected in cucurbit fields in Kaftarak area (Shiraz) and verified by ELISA test, designated Fars isolate (ZYMV-F). The virus was propagated in squash plants under greenhouse conditions and purified from infected tissues. The antiserum obtained from purified virus was used in serological tests. Complete sequence of the virus genome was determined using mRNA capture kit and PCR with specific primers followed by cloning and sequencing. Following the sequence walking technique a contig of 9591 bp (JN183062) representing the whole genome of the virus was generated. Phylogenetic analysis based on the nucleotide and amino acid sequence of the whole genome and individual genes showed that ZYMV-F is closest to Israel and Slovakia isolates. Analysis of partial sequence of CP gene showed that ZYMV isolates in Iran are very similar, with Fars and Karaj isolates clustering in the same clade.
AZARFAR, A., IZADPANAH, K., AFSHARIFAR, A., & MASUMI, M. (2012). PURIFICATION AND THE COMPLETE GENOME SEQUENCE OF ZUCCHINI YELLOW MOSAIC VIRUS- FARS ISOLATE. Iranian Journal of Plant Pathology, 48(3), 403-409.
MLA
A. AZARFAR; K. IZADPANAH; A. AFSHARIFAR; M. MASUMI. "PURIFICATION AND THE COMPLETE GENOME SEQUENCE OF ZUCCHINI YELLOW MOSAIC VIRUS- FARS ISOLATE". Iranian Journal of Plant Pathology, 48, 3, 2012, 403-409.
HARVARD
AZARFAR, A., IZADPANAH, K., AFSHARIFAR, A., MASUMI, M. (2012). 'PURIFICATION AND THE COMPLETE GENOME SEQUENCE OF ZUCCHINI YELLOW MOSAIC VIRUS- FARS ISOLATE', Iranian Journal of Plant Pathology, 48(3), pp. 403-409.
VANCOUVER
AZARFAR, A., IZADPANAH, K., AFSHARIFAR, A., MASUMI, M. PURIFICATION AND THE COMPLETE GENOME SEQUENCE OF ZUCCHINI YELLOW MOSAIC VIRUS- FARS ISOLATE. Iranian Journal of Plant Pathology, 2012; 48(3): 403-409.
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