For taxonomic studies, 37 isolates of the genus Rhizopus obtained from various hosts in Tehran and west Azarbaijan provinces were investigated. Internal transcribed spacer (ITS) region and D1-D2 regions of the large subunit ribosomal DNA were amplified using ITS1/NL4 primer pair. Amplified products were digested with four restriction endonucleases HaeIII, RsaI, HinfI and MspI and seven representative isolates were selected and sequenced. These and 23 isolates from GenBank were analyzed to determine their phylogenetic relationship. In contrast to the extreme variation of the ITS region and D1/D2 regions of LSU rDNA proved to be valuable in the phylogeny of the genus Rhizopus. Based on the results, 37 isolates were divided into three groups, R. lyococcus, R. stolonifer and R. oryzae. R. lyococcus is a new record for Iran which is the first report of R. stolonifer on nectarines and R. oryzae on persimmons and nectarines. R. stolonifer on nectarines is a new record for the world.
MOHAMMADI, P., ETEBARIAN, H., ZARE, R., & EBRAHIMI, M. (2011). MOLECULAR AND PHYLOGENETIC ANALYSIS OF RHIZOPUS ISOLATES BASED ON It’s REGION AND
D1-D2 REGIONS OF rDNA *. Iranian Journal of Plant Pathology, 47(3), 195-205.
MLA
P. MOHAMMADI; H.R. ETEBARIAN; R. ZARE; M. EBRAHIMI. "MOLECULAR AND PHYLOGENETIC ANALYSIS OF RHIZOPUS ISOLATES BASED ON It’s REGION AND
D1-D2 REGIONS OF rDNA *". Iranian Journal of Plant Pathology, 47, 3, 2011, 195-205.
HARVARD
MOHAMMADI, P., ETEBARIAN, H., ZARE, R., EBRAHIMI, M. (2011). 'MOLECULAR AND PHYLOGENETIC ANALYSIS OF RHIZOPUS ISOLATES BASED ON It’s REGION AND
D1-D2 REGIONS OF rDNA *', Iranian Journal of Plant Pathology, 47(3), pp. 195-205.
VANCOUVER
MOHAMMADI, P., ETEBARIAN, H., ZARE, R., EBRAHIMI, M. MOLECULAR AND PHYLOGENETIC ANALYSIS OF RHIZOPUS ISOLATES BASED ON It’s REGION AND
D1-D2 REGIONS OF rDNA *. Iranian Journal of Plant Pathology, 2011; 47(3): 195-205.
)