The ability of endophytic bacteria to inhibit the growth of crown and root rot fungal-like Phytophthora nicotianae and Phytophthora citrophthora on Bakraii rootstock (Citrus sp.)

Document Type : Research Article

Authors

1 Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, University of Hormozgan

2 Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, University of Hormozgan, Iran

3 Phytopathology Department, Bu-Ali Sina University, Hamedan

4 Plant Protection Research Department, Hormozgan Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Bandar Abbas

5 Horticulture crops Research Department, Qazvin Agricultural and Natural Resources Research and Education Center, AREEO, Qazvin

Abstract

Endophytic bacteria are one of the most powerful tools in bio-controlling plant pathogens and protecting plants from damage. The evaluation of inhibitory effect of endophytic bacteria isolated from Mandarin orchards in Hormozgan province against crown and root rot caused by fungal-like Phytophthora nicotianae and Phytophthora citrophthora in laboratory (completely randomized design) and greenhouse (factorial experiment in completely randomized design) was performed with three replications. Also, the biomass parameter of fresh and dry weight of the stem, root and their corresponding lengths were investigated. In this experiment, 17 isolates of endophytic bacteria were identified using 16S rDNA gene. In the laboratory experiment was found that out of 17 endophytic bacteria, 3 isolates had significant inhibitory effect at the 5 percentage probability level; So that in the greenhouse conditions they reduced the disease severity of P. nicotianae and P. citrophthora isolates by 75.5 percentage compared to the control. The sequence of these three isolates was 99 percentage similar to the sequence of Paracrauroccus sp. , Kytococcus schroeteri and Psychrobacillus psychrodurance species. In the greenhouse experiment, the comparison of the means showed that the fresh weight of stem, root and stem length treated with endophytic bacteria increased compared to the control. In general, the endophytic bacteria Paracrauroccus sp. ,Kytococcus schroeteri and Psychrobacillus psychrodurance separately and in combination can be suggested as an effective tool for biological control of the cause of crown and root rot at the Bakraii rootstock.

Keywords


Abraham A., Philip S., Jacob C. K. and Jayachandran K. 2013. Novel bacterial endophytes from Hevea brasiliensis as biocontrol agent against Phytophthora leaf fall disease. Biological Control 58: 675-684.
Ahmadi K., Ebadzadeh H., Hatami F., Hosseinpour R., Ebdeshah H. 2020. Agricultur Statistics, 3st Volume, Horticulture Production. Ministry of Agriculture Planning and Economic Deputy. Information Technology Center Tehran Iran. 156 p.
Akbarpour K. and Banihashemi Z. 1998. Distribution and severity of citrus gummosis in Fars province. Iran. Iranian Journal Plant Pathology. 34: 18–31(in Persian with English Summary).
Alvani S., Rohani H. and Mehdikhani E. 2012. Relationship between hydrogen cyanide and siderophore production by fluorescent Pseudomonads of wheat rhizosphere with growth rate of the plant. Iranian Journal of Plant Protection Science 43:1-12(in Persian with English Summary).
Araújo W. L., Marcon J., Maccheroni W., van Elsas J. D., van Vuurde J. W. and Azevedo J. L. 2002. Diversity of endophytic bacterial populations and their interaction with Xylella fastidiosa in citrus plants. Applied and environmental microbiology 68: 4906-4914.
Backman P.A. and Sikora R.A. 2008. Endophytes: an emerging tool for biological control. Biological control 46: 1-3.
Barka E. A., Gognies S., Nowak J., Audran J. C. and Belarbi A. 2002. Inhibitory effect of endophyte bacteria on Botrytis cinerea and its influence to promote the grapevine growth. Biological Control 24: 135-142.
Banihashemi Z. 1983a. Detection and isolation of Phytophthora spp. In citrus soil and their distribution in citrus growing areas of Southern Iran. Proc. 7th Iran. Plant Protection. Congress. Tehran, Iran. (in Persian with English Summary).
Banihashemi Z. and Fatehi J. 1989. Reaction of cucurbit cultivars to Phytophthora drechsleri and P. capsici in greenhouse. Proceeding of 9th. Iranian Plant Protection Congress. Mashhad. Iran: 89.
Brasier C. M., Sanchez-Hernandez E. and Kirk S. A. 2003. Phytophthora inundata sp. nov., a part heterothallic pathogen of trees and shrubs in wet or flooded soils. Mycological Research 107: 477-484.
Cheplick G. P., Clay K. and Marks S. 1989. Interactions between infection by endophytic fungi and nutrient limitation in the grasses Lolium perenne and Festuca arundinacea. New Phytologist 111: 89-97.
Cho K. M., Hong S. Y., Lee S. M., Kim Y. H., Kahng G. G., Lim Y. P., Kim H. and Yun H. D. 2007. Endophytic bacterial communities in ginseng and their antifungal activity against pathogens. Microbial Ecology 54: 341-351.
Erwin D. C. And Riberio O. K. 1996. Phytophthora diseases worldwide. St. Paul, MN: American Phytopathological Society.
Fotouhi Ghazvini R. and Fattahi Moghadam J. 2006. Citrus cultivation in Iran.2th ed. University of Guilan Publications.
Hassan S. E. D. 2017. Plant growth-promoting activities for bacterial and fungal endophytes isolated from medicinal plant of Teucrium polium L. Journal of advanced research 8: 687-695.
Hornschuh M., Grotha R. and Kutschera U. 2002. Epiphytic bacteria associated with the bryophyte Funaria hygrometrica: effects of Methylobacterium strains on protonema development. Plant Biology 4: 682-687.
Ippolito A., Decicco V., Cicco E. and Salerno M. 1990. Role of Phytophthora spp. in citrus decline in Apulia and Basilicata, Italy. Bulletin EPPO 20: 91-94.
Iswandi A., Bossier P., Vandenabeele J. and Verstraete W. 1987. Effects of seed inoculation withthe rhizopseudomonas strain 7NSK2 on the root microbiota of maize and barley. Biology and Fertility ofSoil 3: 153-158.
Gilardi G., Demarchi S., Gullino M. L. and Garibaldi A. 2014. Control of phytophthora nicotianae of tomato by using non-conventional strategies. In VIII International Symposium on Chemical and Non-Chemical Soil and Substrate Disinfestation 1044: 325-330.
Grech N. M. and Rijkenberg F. H. J. 1992. Injection of electrolytically generated chlorine into citrus microirrigation systems for the control of certain waterborne root pathogens. Plant disease 76: 457-461.
McInroy J. A. and Kloepper J.W. 1995. Survey of indigenous bacterial endophytes from cotton and sweet corn. Plant and soil 173: 337-342.
Najafiniya M., Azadvar M. and Jahanshahiafshar F. 2020. Evaluation the Population of Citrus Root Nematode (Tylenchulus semipenetranse Cobb, 1913) and semi fungus Phytophthora nicotianae (Breda de Hann, 1896) in Healthy and Declining citrus Trees in south of Kerman province. Journal of Applied Research in Plant Protection8: 29-46 (in Persian with English Summary).
Pal K. K. and Gardener B. M. 2006. Biological control of plant pathogens. The Plant Health Instructor.
Rasolisedghiyani M., Rahimiyan P., Khavari K., and Malakoti M. H. 2005. Study of population density and identification of wheat phizospheric fluorescent pseudomonas in different region in Iran. Journal of Water and Soil Science 19:224-234.
Reis V. M., Baldani J. I., Baldani V. L. D. and Dobereiner J. 2000. Biological dinitrogen fixation in gramineae and palm trees. Critical Reviews in Plant Science 19: 227-247.
Safdar A., Javed N., Khan S. A., Khan H. U., Rehman A. and Haq I. U. 2010. Survey and investigation of different citrus growing areas for citrus sudden death syndrome. Pakestanian Jornal of Phytopathology 22: 71-78.
Safdarpour F. and Khodakaramian G. 2019. Assessment of antagonistic and plant growth promoting activities of tomato endophytic bacteria in challenging with Verticillium dahliae under in-vitro and in-vivo conditions. Biological Journal of Microorganism 7: 77-90.
Sambroo K.J. and Russell D.W. 2001. Molecular cloning a laboratory manual. Third
edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York: 502- 510.
Sandler H. A., Timmer L. W., Graham J. H. and Zitko S. E. 1989. Effect of fungicide applications on populations of Phytophthora parasitica, and on feeder root densities and fruit yields of citrus trees. Plant Disease. 73: 902-906.
Siddiqui Y., and Meon S. 2009. Effect of seed bacterization on plant growth response
and induction of disease resistance in chili. Agricultural Sciences in China 8: 963-971
Shekari A., Banihashemi S. Z., naserian E., Saboh Roh Monfared A .2011.Distribution, popopulation density, and virulence of citrus Gummosis and brown rot in Mazandaran province. Iranian Journal of Plant Pathology 48: 39-53.
Shi J., Liu A., Li X., Feng S. and Chen W. 2011. Inhibitory mechanisms induced by the endophytic bacterium MGY2 in controlling anthracnose of papaya. Biological Control 56:.2-8.
Song Y. C., Li H., Ye Y.H., Shan C. Y., Yang Y. M. and Tan R. X. 2004. Endophytic naphthopyrone metabolites are co-inhibitors of xanthine oxidase, SW1116 cell and some microbial growths. FEMS Microbiology Letters 241:.67-72.
Sturz A. V. and Nowak J. 2000. Endophytic communities of rhizobacteria and the strategies required to create yield enhancing associations with crops. Applied soil ecology 15: 183-190.
Waterhouse G. M. 1963a.Key to the species of Phytophthora de Bary. Mycological Paper 92: 30pp.
Waterhouse G. M. 1970b.The Genus Phytophthora deBary. Mycological Paper12: 259 pp.
Waterhouse G. M., Newhook F. J. and Stamps D. J. 1983c.Present criteria for classification of Phytophthora. pp:139-147.
Whipps J.M. 2001. Microbial interactions and biocontrol in the rhizosphere. Journal of experimental Botany 52: 487-511.
Yang X. and Hong C. 2020. Biological control of Phytophthora blight by Pseudomonas protegens strain 14D5. European Journal of Plant Pathology 156(2): 591-601.
Tan R. X. and Zou W. X. 2001. Endophytes: a rich source of functional metabolites. Natural product reports 18: 448-459.
Zhang B., Salituro G., Szalkowski D., Li Z., Zhang Y., Royo I., Vilella D., Dı́ez, M. T., Pelaez F., Ruby C. and Kendall R. L. 1999. Discovery of a small molecule insulin mimetic with antidiabetic activity in mice. Science 284: 974-977.
Zouaoui M., Essghaier B., Weslati M., Smiri M., Hajlaoui M. R. and Zouaoui N. S. 2019. Biological control of clementine branch canker caused by Phytophthora citrophthora. Phytopathologia Mediterranea 58: 547-558.