The first report of the Chilli leaf curl virus and its beta satellite from bell peppers and tomatoes from the central provinces of Iran

Document Type : Short Article

Authors

1 PhD Student of Plant Pathology, Faculty of Agriculture, Shiraz University

2 Professor, Plant Virology Research Center, College of Agriculture, Shiraz University, Shiraz, Iran.

3 Assistant professor of plant pathology, Department of Agriculture, Payame Noor University, Thehran, Iran.

Abstract

During a survey in 2021 a number of pepper and tomato plant samples with the begomovirus-like symptoms such as crinkled and curling leaves were collected from pepper and tomato greenhouses in three central provinces of Iran (Yazd, Isfahan and Kerman). Some weed species occurring within proximity to the greenhouse cultivated tomato crops were also sampled. To identify the Begomovirus infection in the collected samples, total DNA samples were extracted using a CTAB-based method and subjected to polymerase chain reaction (PCR)using the begomovirus degenerate primer pair BC primer and primer181V (Anfoka et al. 2005). This primer pair produced an amplicon of about 500 bp from pepper (Yazd and Kerman), tomato (Yazd) and puppet (Yazd) samples. Multiple alignment of the resulted nucleotide sequences showed that the isolates share maximum and minimum identities of 97.8 to 88.4% respectively with nucleotide sequences of chili leaf curl virus (ChiLCV) isolates reported from Oman (97.83%), Pakistan (97.83%), and India (97.64%) while they showed less than 80.2% similarity with other geminiviruses. Furthermore, a 1351bp fragment of DNA-β satellite genome was successfully amplified from pepper and tomato isolates (collected in Molabashi and Dehaghan regions in Yazd and Isfahan provinces respectively), using specific beta-satellite primer pair Beta01 / Beta02 (Cui et al., 2004). Alignment of the obtained sequences showed a very close homology to the beta satellite (ToLCB) associated with Iranian isolate of ToLCV, (MN175237.1) (Benanej et al. 2019).

Keywords

References

Anfoka G. H. Abhary M. & Nakhla M. K. 2005. Molecular identification of species of the Tomato yellow leaf curl virus complex in Jordan. Journal of Plant Pathology. 65-70.
Bananej K. Shafiq M. & Shahid M. S. 2021. Association of cotton leaf curl Gezira virus with tomato leaf curl betasatellite infecting Carica papaya in Iran. Australasian Plant Disease Notes. 16(1), 1-4.
Briddon R. W. Mansoor S. Bedford I. D. Pinner M. S. Saunders K. Stanley J. Markham P. G. 2001. Identification of DNA components required for induction of cotton leaf curl disease. Virology. 285(2), 234-243.
Cui X. Tao X. Xie Y. Fauquet C. M. & Zhou X. 2004. A DNAβ associated with Tomato yellow leaf curl China virus is required for symptom induction. Journal of virology. 78(24), 13966-13974.
Chattopadhyay B. Singh A. K Yadav T. Fauquet C. M. Sarin N. B. Chakraborty S. 2008. Infectivity of the cloned components of a begomovirus: DNA beta complex causing chilli leaf curl disease in India. Archive of Virology. 153:533–539.
Deng D. McGrath P. F. Robinson D. J. & Harrison B. D. 1994. Detection and differentiation of whitefly‐transmitted geminiviruses in plants and vector insects by the polymerase chain reaction with degenerate primers. Annals of applied Biology 125(2), 327-336.
Gawel N. J. 1991. A modified CTAB DNA extraction procedure for Musa and Ipomea. Plant Molecular Biology Report, 9, 292-296.
Nigam K, Suhail S, Verma Y, Singh V, Gupta S (2015) Molecular characterization of begomovirus associated with leaf curl disease in chilli. World Journal of Pharm Research. 4:1579–1592.
Pakniat A. Behjatnia S. A. A. Kharazmi S. Shahbazi M. & Izadpanah K. 2011. Molecular characterization and construction of an infectious clone of a new strain of Tomato yellow leaf curl virus in southern Iran.
Rojas M. R. 1993. Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminiviruses. Plant Disease. 77, 340-347.
Senanayake DMJB, Mandal B, Lodha S, Varma A (2007) First report of Chilli leaf curl virus affecting chilli in India. Plant Pathology Journal. 56:343.
Iranian Journal of Plant Pathology
Volume 57, Issue 4
December 2022
Pages 337-341

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