Sleeping hibiscus (Malvaviscus penduliflorus) plants showing leaf spot symptoms have been observed since 2008 in Sari. The spots were 2-4 mm in diameter, necrotic angular to irregular brown to black, and surrounded by chlorotic halos. A levan positive, Gram and oxidase negative bacterium was isolated from the symptomatic leaves on sucrose nutrient agar (NAS). The bacterium produced fluorescent pigment on medium B of King and rotted potato tuber slices. Pathogenicity of selected strains was confirmed by inoculation of sleeping hibiscus, sweet orange and seville orange with bacterial suspension of strains. In electrophoretic profile of cell proteins the strains were, partially similar to the strains of Pseudomonas viridiflava. 16S rRNA gene of strains were sequenced at Millegen company(France) using standard primers , nucleotide sequence analysis in GenBank showed that the nucleotide sequence of 16s rRNA gene of sleeping hibiscus strains had 99% similarity to few reference strains of Pseudomonas viridiflava. In ERIC-BOX and REP- PCR, the fingerprints of the strains from sleeping hibiscus showed 45% and in Is50- PCR 46% similarity to the reference strain of Pseudomonas viridiflava. Based on phenotypic, pathogenicity and genomic properties, the causal agent of leaf spot of sleeping hibiscus (Malvaviscus penduliflorus) were identified as levan positive strains of Pseudomonas viridiflava
ROUHRAZI, K., & RAHIMIAN, H. (2011). BACTERIAL LEAF SPOT OF Malvaviscus penduliflorus INCITED BY LEVAN-POSITIVE STRAINS OF Pseudomonas viridiflava. Iranian Journal of Plant Pathology, 47(3), 249-262.
MLA
K. ROUHRAZI; H. RAHIMIAN. "BACTERIAL LEAF SPOT OF Malvaviscus penduliflorus INCITED BY LEVAN-POSITIVE STRAINS OF Pseudomonas viridiflava". Iranian Journal of Plant Pathology, 47, 3, 2011, 249-262.
HARVARD
ROUHRAZI, K., RAHIMIAN, H. (2011). 'BACTERIAL LEAF SPOT OF Malvaviscus penduliflorus INCITED BY LEVAN-POSITIVE STRAINS OF Pseudomonas viridiflava', Iranian Journal of Plant Pathology, 47(3), pp. 249-262.
VANCOUVER
ROUHRAZI, K., RAHIMIAN, H. BACTERIAL LEAF SPOT OF Malvaviscus penduliflorus INCITED BY LEVAN-POSITIVE STRAINS OF Pseudomonas viridiflava. Iranian Journal of Plant Pathology, 2011; 47(3): 249-262.
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