نوع مقاله : مقاله کامل پژوهشی
نویسندگان
1 هیات علمی
2 بخش گیاهپزشگی، دانشکده کشاورزی،دانشگاه شیراز،شیراز، ایران
3 گروه بیوتکنولوژی دارویی، دانشکده داروسازی،دانشگاه علوم پزشکی شیراز، شیراز، ایران.
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Identifying resistance related metabolites as biomarker for screening wheat germplasms resistant to leaf rust (Puccinia triticina) is a useful approach to increase the speed, accuracy and cost effectiveness of screening disease resistance. The relatively polar metabolites were extracted in a mixture of methanol-water from near isogenic line (NILs) of wheat susceptible (Thatcher Lr22b) or resistant (Thatcher Lr25) to leaf rust in the two leaf stage, 24 h after inoculation with the pathogen, talcum powder or sterilized distilled water, in three replications. Metabolites were analyzed using a GC/MS. ANOVA showed that abundance of 26 metabolites had significant differences among experimental factors including near isogenic lines (NILs), inoculation (I) and NILs ×I interaction. Abundance of 26 significant metabolites was subjected to canonical discriminant analysis and hierarchical cluster analysis to group observations of metabolite profiles based on similarities and differences in the investigated metabolic space. Finally 13 resistance related metabolites (RR) and 13 Pathogenesis related metabolites (PR) were identified that the majority of RR metabolites involved in induced resistance and constitutive resistance. Significant metabolites included metabolites with potential signaling and/or antimicrobial activity as well as molecules used as precursors for cell wall reinforcement, phenolic and alkaloids compounds, and accumulation of amino acids and proteins associated with resistance. Up-regulation of the number pathways upon infection of wheat to leaf rust fungus is discussed.
Key words: metabolic profiling, Wheat leaf rust, screening resistance, gas chromatography/mass spectrometry.
کلیدواژهها [English]
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