ارزیابی برخیاز فاکتورهای بیماریزایی و مرتبط با پرآزاری استرینهای Xanthomonas arboricola pv. pruni در استان خراسانرضوی

نوع مقاله: مقاله کامل پژوهشی

نویسندگان

چکیده

باکتری(Xap) Xanthomonas arboricola pv. pruniعامل لکه روی برگ، میوه و شانکر‍باکتریایی شاخه در شرایط گرم و مرطوب روی درختان میوه‍هسته دار می‍باشد. در تحقیق حاضر، بیماریزایی 18 استرین Xap خراسان‍رضوی درشرایط گلخانه روی نهال‍های آلوی سانتاروزا بررسی‍شد. تعدادی از فاکتورهای بیماریزایی شامل پلاسمید pXap41، افکتورهای اختصاصی تیپ III ترشحی و همچنین تعدادی ویژگی دخیل در پرآزاری از‍جمله تحرک، تولید بیوفیلم، زانتان، بیوسورفکتانت، آنزیم‍های تجزیه‍کننده دیواره‍سلولی و ارتباط احتمالی آنها با میزان بیماریزایی استرین‍ها بررسی شدند. بر‍اساس درصد لکه‍های نکروز، استرین‍ها در دوگروه بیماریزایی قوی و ضعیف قرار‍گرفتند. تمامی ۱8 استرین مورد‍مطالعه به‍همراه استرین استاندارد ICMP7485 ، پلاسمیدpXap41 وژن‍های افکتوری اختصاصیxopE3 وxopA را دارا‍بودند. در برخی موارد بین شدت بیماریزایی استرین‍ها و فاکتورهای دخیل در پرآزاری ارتباط مستقیم وجود داشت. برای مثال دو استرین ShL45 و NB28k با بالاترین درصد لکه‍های نکروز (5/62 درصد)، بیوسورفاکتانت و بیوفیلم بیشتری تولید نمودند و اختلاف معنی‍داری با سایر استرین‍ها در سطح احتمال پنج درصد نشان دادند. به‍نظر می‍رسد زانتان در پرآزاری استرین‍های Xap نقش موثری نداشته باشد. میزان حرکت توده‍ای در 3/92 درصد جدایه‍های گروه بیماریزایی قوی، بیشتر‍از حرکت شناوری بود (05/0 ≥P). همچنین، تمامی استرین‍ها قدرت ترشح آنزیم‍های پروتئاز، سلولاز، پلی‍گالاکتوروناز و پکتات‍لیاز را با مقادیر متفاوت داشته اما میزان ترشح همه آنزیم‍ها با پرآزاری استرین‍ها همخوانی کامل نداشت. برای دستیابی‍به نتایج دقیق‍تر، ارزیابی استرین‍های Xap بیشتر از مناطق مختلف کشور به‍همراه سایر پاتووار‍های گونه X. arboricola پیشنهاد می‍شود.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of some pathogenicity and virulence factors in Xanthomonas arboricola pv. pruni strains from Khorasan-Razavi Province

نویسندگان [English]

  • A. Rahmanzadeh
  • S. Baghaee-Ravari
  • E. Mahdikhani-Moghaddam
چکیده [English]

Xanthomonas arboricola pv. pruni (Xap) is a causal agent of leaf, fruit spot, and branch bacterial canker in warm and humid conditions on stone fruit trees. Herein, the pathogenicity of 18 Xap strains from Khorasan-Razavi Province, Iran was evaluated in greenhouse trials on Santha-Rosa plum saplings. Some pathogenicity factors including pXap41, T3SS effectors, and virulence features such as motility, biofilm, xanthan and biosurfactant production, cell wall degrading enzymes and their possible correlation with pathogenicity rate were investigated. The studied strains were divided into two severe and weak pathogenicity groups based on percentage of necrotic lesions. All 18 strains besides ICMP7485, had pXap41, xopE3 and xopA effector genes. In some cases, there was a direct correlation between disease severity and factors involving in virulence. For example, two strains, ShL45 and NB28k, showed the highest percentage of necrosis spots (62.5%), produced high levels of biosurfactant and biofilm that indicated significant differences with other strains at 5% probability levels. It seems that xanthan has no significant effective role in Xap virulence. The rate of swarming in 92.3 % of strains belonging to the severe pathogenicity group was more than the swimming (P≤ 0.05). All strains were also capable of secreting different amounts of enzymes, including protease, cellulase, polygalactronase, and pectatelyase. However, the rate of secretion in all them was not entirely consistent with virulence. It is suggested that more Xap strains from different regions of the country, along with other X. arboricola pathovars, should be tested to get results that are more accurate.

کلیدواژه‌ها [English]

  • Plasmid pXap41
  • Virulence
  • Xanthomonas arboricola pv. pruni
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