عنوان مقاله [English]
In order to identify the plant-parasitic nematodes associated with medicinal plants in Boyer-Ahmad region, Iran, 84 soil and root samples were collected during 2015-2016. The nematodes were extracted from soil samples, according to the sieving-centrifugal flotation method, and then fixed and transferred to glycerin according to the modified De Grisse’s method. Identification of the nematodes insides the roots was also done with the Hooper’s staining technique. After microscopic observations of the permanent slides, the necessary measurements were made. Finally, the extracted nematodes were identified at species level using available diagnostic keys. In this study, 28 species belong to 14 genera were identified as follow:
Amplimerlinius globigerus, Aphelenchoides cyrtus, A. haguei, A. varicaudatus, Helicotylenchus digitiformis, H. digonicus, H. exallus, H. pseudorobustus, H. varicaudatus, H. vulgaris, Longidorus africanus, Meloidogyne hapla, M. javanica, Merlinius brevidens, Mesocriconema antipolitanum, M. lobellum, Nagelus obscurus, Pratylenchoides ritteri, Pratylenchus cruciferus, P. curvicauda, P. delattrei, P. neglectus, P. sefaensis, P. sudanensis, P. thornei, Rotylenchus cypriensis, Scutylenchus rugosus, Xiphinema pachtaicum, Zygotylenchus guevarai.
Mesocriconema lobellum and Pratylenchus sudanensis were recovered from the rhizosphere of marsh-mallow and horehound plants, respectively and are reported for the first time from Iran here.
Dastar A., Mohamadian KH. M., Bagherizadeh L., Sheikhi K., Namjoo K. and Kazemi M. R. 2015. Geography of governorates. Gavernoratology of Kohgilooye and Boyer-Ahmad. Iran Publicatoin. 4: 90. (In Persian)
De Grisse A. T. 1969. Redescription ou modification de quelques techniques utilisées dans 1ʼétude des nématodes phytoparasitaires. Medelingen van de Rijksfaculteit Landbow Wetenschappen Gent 34: 351-369.
Fallah-Hoseini H., Fakhrzadeh H., Larijani B. and Shikhsamani A. H. 2006. Review of anti-diabetic medicinal plant used in traditional medicine. Journal of Medicinal Plants 5: 1–8. (In Persian with English Summary)
Geraert E. 2013. The Pratylenchidae of the world: Indentification of the family Pratylenchidae (Nematoda: Tylenchida). Academia Press, Gent, Belgium. 430 p.
Haldar K. and Gantait V. V. 2016. Diversity and distribution of plant parasitic nematodes associated with medicinal plants in West Bengal, India. Journal of Environment and Sociobiology 13(1): 75-79.
Hartman K. M. and Sasser J. N. 1985. Identification of Meloidogyne species on the basis of differential host and perineal-pattern morphology, pp. 69-77. In: K. R. Barker., C. C. Carter and J. N. Sasser (Eds.), An advanced treatise on Meloidogyne. A Cooperative publication of the department of plant pathology and the United States agency for international development. North Carolina State University Graphics, Raleigh, North Carolina. USA.
Hooper D. J. 1986. Preserving and staining nematodes in plant tissues, pp. 81-85. In: J. F. Southey [Ed.], Laboratory methods for work with plant and soil nematodes. Ministry of Agriculture, Fisheries and Food, Her Majesty's Stationery Office, London, UK.
Karl A. C., Souza R. M. and Mattos J. K. A. 1997. Patogenicidade de Meloidogyne javanica em quatro especies de plantas medicinais. Horticultura Brasileira 15(2): 118-121.
Loof A. A. and Yassin A. M. 1970. Three new plant-parasitic nematodes from the Sudan, with notes on Xiphinema basiri Siddiqi, 1959. Nematologica 16: 537-546.
Mountain W. B and Patrick Z. A. 1959. The peach replant problem in Ontario. VII. The pathogenicity of Pratylenchus penetrans (Cobb, 1917) Filip & Stek. 1941. Canadian Journal of Botany 37: 459-470.
Nasresfahani M., Mohsenzadeh Kermani A., Zargani M. and Alizadeh M. 2015. Evaluation nematode of some medicinal plant in Isfahan. Indian Journal of Fundamental and Applied Life Sciences 5: 2665-2674.
Oka Y., Kolti H. and Bar-Eyal M. 2000. New strategies for the control of plant parasitic nematodes. Pest Management Science 56: 983-988.
Park S. D., Kim J. C. and Khan Z. 2004. Host status of medicinal plants for Meloidogyne hapla. Nematropica 34: 39-43.
Pramodini M., Mohilal N. and Gambhir R. K. 2007. Criconemella lobella sp. n. and Caloosia langolus sp.n. from lemon plants of Manipur. Flora and Fauna 13: 433-438.
Razzaz Hashemi S. R. and Akbarinia A. 2008.Responses of some medicinal plants to root-knot nematode Medoidogyne javanica under greenhouse conditions. 18th Iranian Plant Protection Congress, 24-27 August 2008. Hamadan. P 561.
Salehi F., Pourjame E., Taheri1 A. and Jahanshahy Afshar F. 2010.Identification Tylenchida with medicinal plants in Tehran and Golestan Provinces. 19th Iranian Plant Protection Congress, 31 July-3 August 2010. Iranian Research Institute of Plant Protection. P 585.
Seinhorst J. W. 1959. A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica 4: 67-69.
Sivakumar M. and Vadivelu S. 1997. Parasitic nematodes associated with medicinal and aromatic plants. Indian Journal of Nematology 27: 58-62.
Sultan M. S., Sharma S. K. and Dhillon N. K. 2010. Identification of nematode problems in medicinal, aromatic and spice plants in Punjab, India. Trends in Biosciences 3(1): 56-57.